This mixture is then incubated at 55°C for one particular hour. Then the sample is phenol-extracted once with phenol/chloroform/isoamyl alcohol Resolution and centrifuged. The aqueous layer is eradicated to a clean microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a second time. After the pellet is dried, https://www.pinterest.com/pin/993958580252568734/